Researchers develop novel DNA biosensor for early analysis of cervical most cancers


Chung-Ang University researchers develop novel DNA biosensor for early diagnosis of cervical cancer
Chung-Ang College researchers have developed a novel electrochemical nano-onion/molybdenum disulfide nanosheet composite-based DNA biosensor that effectively and particularly detects human papillomavirus (HPV)-16 and HPV-18, enabling early analysis of cervical most cancers. Credit score: Journal of Nanobiotechnology

Molybdenum disulfide (MoS2) has lately garnered consideration amongst supplies science researchers owing to its capability to kind two-dimensional nanosheets like graphene. The nanosheets are created by the stacking of S–Mo–S layers interacting by way of Van der Waals interactions.

Moreover, the distinctive structural, optical, thermal, and electrochemical properties of MoS2 have opened up a number of analysis avenues throughout a number of fields, together with the event of biomolecule sensing and chemical detection platforms, optoelectronics, supercapacitors, and batteries.

Historically, carbon nanostructures have been employed as an immobilization platform for DNA. In an effort to substitute carbon with MoS2 as an efficient electrochemical DNA sensor, {the electrical} conductivity of MoS2 must be improved significantly.

Towards this backdrop, Affiliate Professor Eunah Kang and Mr. Youngjun Kim from the Faculty of Chemical Engineering and Materials Science at Chung-Ang College, Korea have lately give you a chic answer. The duo has developed an electrochemical DNA biosensor utilizing a graphitic nano-onion/molybdenum disulfide (MoS2) nanosheet composite, which successfully detects human papillomavirus (HPV)-16 and HPV-18, and might function an early analysis of cervical most cancers.

“Nano-onions possess graphitic sp2 buildings and are derived from crystalline sp3nanodiamonds by way of thermal annealing or laser irradiation,” explains Dr. Kang. Their breakthrough was revealed within the Journal of Nanobiotechnology.

The researcher duo ready the novel electrode floor for probing DNA chemisorption by enabling chemical conjugation between two useful teams: acyl bonds on the surfaces of functionalized nano-onions and amine teams current on the modified MoS2 nanosheets.

Cyclic voltammetry experiments revealed {that a} 1:1 composite electrode had an improved rectangular form in comparison with that of an MoS2 nanosheet electrode. “This indicated the amorphous nature of the nano-onions with curved carbon layers that facilitated an enhancement in digital conductivity in comparison with MoS2 nanosheet alone,” highlights Dr. Kang.

Moreover, the duo measured the sensitivity of their novel electrochemical DNA biosensor system in the direction of HPV-16 and HPV-18 by using differential pulse voltammetry (DPV) method within the presence of methylene blue (MB) as a redox indicator. Dr. Kang says, “The DPV present peak was lowered after probe DNA chemisorption and goal DNA hybridization. Because the hybridized DNA was double-stranded, it induced much less efficient MB electrostatic intercalation, leading to a decrease oxidation peak.”

The duo discovered that, in comparison with the MoS2 nanosheet electrode, the nano-onion/MoS2 nanosheet composite electrode attained increased present peaks, indicating a better change within the differential peak. This was attributed to an enhanced conductive electron switch owing to the nano-onion.

Notably, the goal DNAs produced from HPV-16 and HPV-18 Siha and Hela most cancers cell strains had been detected by the proposed sensor successfully and with excessive specificity. Consequently, MoS2 nanosheets with improved electrical conductivity facilitated by complexation with nano-onions gives an acceptable platform for creating efficient and environment friendly electrochemical biosensors for the early analysis of all kinds of illnesses, together with cervical most cancers.

Moreover, combining nano-onions or nanodiamonds with completely different natural biomaterials can facilitate chemical performance, electron switch conductivity, mild absorption, and extra. These, in flip, can result in progressive illness sensing, focused drug supply techniques, and biomedical imaging and diagnostics.

Extra info:
Youngjun Kim et al, A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors, Journal of Nanobiotechnology (2023). DOI: 10.1186/s12951-023-01948-6

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Researchers develop novel DNA biosensor for early analysis of cervical most cancers (2023, September 7)
retrieved 8 September 2023

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